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PhD position: "Plastisphere Microbiome and Microplastics Ecotoxicity in the ViBRANT SEA-project"

On-site
  • 't Horntje, Texel, Noord-Holland, Netherlands
Marine Microbiology & Biogeochemistry (MMB)

Job description

Validating Biodegradation Rates and Reactions Applying Novel Technologies and Systems Ecology Approaches (ViBRANT-SEA) is an Advanced European Research Council (ERC) supported project that is recruiting a PhD Candidate (1.0 FTE). This position is vacant within our Department of Marine Microbiology and Biogeochemistry (MMB) of the Royal Netherlands Institute for Sea Research (NIOZ) and in collaboration with the Institute for Biodiversity and Ecosystem Dynamics (IBED) Department at the University of Amsterdam (UvA) and the Utrecht University’s Faculty of Veterinary Medicine (UUFVM). The successful candidate will be enrolled as an UvA student but work initially at the UUFVM and then at NIOZ’s on Texel.


THE DEPARTMENT

The Department of Marine Microbiology and Biogeochemistry (MMB) is located on Texel. The MMB Department studies microbial communities in all types of marine environments, from estuaries to the deep ocean, from the deep past to the present. Using field and expermental approaches and a variety of analytical, (bio)chemical and genomic techniques, we study the diversity, evolution, physiology and activity of eukaryotes, bacteria, archaea, and viruses in diverse marine environmental settings. We do so by a variety of analytical chemical, biochemical and modern genomic techniques. The MMB Department is well-equipped with laboratories for molecular analyses and state-of-the-art bioinformatics facilities. The MMB technical and scientific staff is international, diverse, and has approximately 80 members, including PhD students, postdocs, and Master students from more than 15 different countries. A group of sixteen staff scientists and fifteen research assistants (junior and senior) form its support core.


THE
UUFVM

Utrecht University’s Faculty of Veterinary Medicine (UUFVM) is the only Veterinary School in the Netherlands and belongs to the international top in education, research and veterinary medical patient care. UUFVM is one of the few European veterinary schools accredited by the American and Canadian Veterinary Medical Associations and the European Association of Establishments for Veterinary Education. The faculty employs approximately 1,000 veterinarians, scientists and support staff and 1,500 students. Its research functions are coordinated by the Institute of Veterinary Sciences and the Graduate School of Life Sciences.


THE ViBRANT-SEA PROJECT

Plastic Marine Debris (PMD) is now considered a planetary boundary threat and the

“Plastic Cycle” a biogeochemical cycle. The impacts of single-use plastic litter in marine environments have prompted a search for greener alternatives such as the biodegradable/ compostable plastics polyhydroxyalkanoates and polylactic acid already being used for consumer goods. The diversity of microbes, their enzymes, and their role in influencing the rates of plastic biodegradation in nature remains poorly characterized – particularly in cold or low-oxygen marine waters and seafloor, where most PMD accumulates. The impact of lag duration, pH, hydrostatic pressure, and potential priority effects of initial microbial colonizers remain vital but unaddressed concerns. In addition, the impact of plastic, its breakdown products, and additives on marine organisms and ecosystems is largely unquantified.

Two urgent questions are how long does plastic last in the marine environment and what are the impacts on ecosystems. This technology-enabled project aims to quantify biodegradation rates of biodegradable plastics and plastic-associated additives in the field and under controlled laboratory conditions, and to develop a fish model system for toxicity tests to fill these knowledge gaps. This project will generate novel microbial metagenomes from global plastic samples collected during cruises and from biodegradation experiments to create a comprehensive list of genes and enzymes linked to biodegradation of biodegradable plastic in marine environments. It will be the first to combine in situ respirometry, stable isotope probing, and advanced imaging techniques to measure biodegradable plastic biodegradation rates, while visualizing microbe-microbe interactions on the plastic surface, revealing cellular mechanisms occurring during active biodegradation.

Finally, it will develop the first marine fish organoid model for consistent and efficient testing of toxicity due to plastics and additives.

Job requirements

THE CANDIDATE

We seek a highly motivated candidate with the ambition to become a scientist as a team member with a background in cell isolation and culturing that has recently actively participated in organoid-related studies with experience in in vitro modelling. In this PhD-trajectory you will be jointly supervised by Prof. dr. L.A. Amaral-Zettler (NIOZ/UvA) and Dr. B. Spee (UUFVM/Regenerative Medicine Center Utrecht). The ideal candidate will have a master’s degree in biochemistry, molecular biology, regenerative medicine or biofabrication and experience in cell culturing, ecophysiology and ecotoxicology. The candidate must be willing to work with and sacrifice limited numbers of wild caught marine fish (sea bass) until the organoid cell line is established and take part in field work in coastal areas and on our open-ocean research vessel. The international character of the institute and department require that the candidate possesses excellent English oral and written communication skills. The key responsibilities for this position are:

  • Isolate novel members of the Plastisphere Microbiome and Marine PHA Cycle via In Situ Chemotaxis Assays (ISCAs) from diverse marine habitats
  • Culture microbes from the gut microbiome of the European Sea Bass Dicentrarchus labrax
  • Construct gut organoids of Dicentrarchus labrax to develop an animal-free model system and characterization thereof
  • Design experiments to test ecotoxicity of biodegradable and non-biodegradable plastics using the marine fish organoid model platform
  • Perform RNASeq experiments using the marine fish organoid model platform
  • Work with technical staff at UvA’s Environmental Chemistry Laboratory and Utrecht University’s NanoSIMS facility as needed to study effects of plastics, additives and microbiome interactions


WHAT WE EXPECT

  • A positive attitude, open mind, and willingness to learn and take on new challenges
  • A highly organized, strong work ethic, excellent documentation skills, and attention to detail
  • Experience with cell culturing and organoid culture
  • Documented microscopy skills and eagerness to learn advanced imaging methods
  • Ability to perform field work and join a research expedition
  • Willingness to be working at Utrecht University during the organoid development stages of the research (year 1) and work on the NIOZ location on Texel for downstream work
  • Flexibility in adapting to the needs of the project and other team members
  • Efficiency and accuracy working independently and as part of the ViBRANT-SEA team

WHAT WE OFFER

  • A supervised PhD-trajectory of 4 years, in which we support you to become a full blown scientist
  • Opportunities to take courses to enhance skills needed for the PhD program
  • Interactions with researchers with backgrounds from a broad range of environmental microbiological topics
  • Excellent access to state-of-the-art culturing, microscope, and environmental chemistry (UvA) facilities
  • Support from an enthusiastic research team composed of your advisors, 2 other ViBRANT-SEA PhD candidates, a full-time data analyst, and a lab manager.
  • Opportunities to assist in teaching undergraduates and mentoring master students


CONDITIONS

  • A temporary contract for 40 hours a week for the duration of 4 years at Royal NIOZ is by NWO-I. The initial contract will be for a period of 1 year and after satisfactory Go-No Go -evaluation in the 9th month, it will be extended for the total duration of 4 years. This should lead to a dissertation (PhD thesis). We will draft an educational plan that includes attendance of courses and scientific meetings.
  • Salary compliant with CAO-WVOI  (Collective Labour Agreement for Dutch Research Institutes) 
  • 338 annualized holiday hours for a full-time 40-hour work week.
  • Pension scheme via ABP, 8% holiday allowance and a year-end bonus of 8.33%. 
  • Employment benefits plan to exchange a portion of your salary for days off or vice versa or can be used to purchase a bicycle with tax benefits.
  • We offer relocation expenses for employees coming from abroad and support with finding accommodation.


APPLICATION

If this profile matches your background and skills, and you are interested in the job, we look forward to receiving your application. You can apply online via the button below. Applications should include the following information:

  • a detailed CV including the months (not just years) when referring to your education and work experience
  • a letter of motivation
  • the names and email addresses of three references willing to write a letter of recommendation

Only complete applications received within the response period via the link below will be considered. We want to be a transparent institute with a healthy working climate and an inclusive culture, where people from diverse backgrounds and gender bring their talents and further develop these talents. We aim for inclusive decision-making processes and expect our leadership to show visible commitment, awareness of bias, and cultural intelligence.


For additional information about this vacancy, please contact Prof. Linda Amaral-Zettler (ViBRANT-SEA Principal Investigator) for substantial questions regarding the position or project. For all procedural question please email to working@nioz.nl


The closing date for this vacancy is 1 June 2024. The interviews will be held on June 3 and 4 (online) and June 6 and 7 on site at NIOZ.

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